De Novo Peptide Sequencing Reveals Many Cyclopeptides in the Human Gut and Other Environments
B. Behsaz, . Mohimani, A. Gurevich, A. Prjibelski, M. F. Fisher, L. Smarr, P. C. Dorrestein, J. S. Mylne, and P. A. Pevzner. Cell Systems 40. (2020).
Abstract
We present a computational pipeline for automated analysis and discovery of cyclic and branch cyclic peptides (cyclopeptides). Cyclopeptides represent an important class of bioactive natural products that includes many antibiotics and anti-tumor compounds. Our Cyclo Novo algorithm for de novo cyclopeptide sequencing of spectral datasets reconstructed many previously unreported cyclopeptides (to the best of our knowledge) that were validated with matching transcriptomics and genome or metagenome mining. Cyclo Novo revealed several cyclopeptides from food that survived the complete human gastrointestinal tract, raising the question of how these compounds might affect the human microbiome.